Molecular cloning

Results: 888



#Item
121Plasmid / Molecular cloning / Restriction enzyme / Vector / TAL effector / Restriction digest / Polymerase chain reaction / Gateway Technology / Subcloning / Biology / Molecular biology / Biochemistry

  Golden Gate TALEN assembly This is an expanded and slightly modified TAL assembly protocol published in the original form in Cermak, et al., 2011 (http://dx.doi.orgnar/gkr218)

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Source URL: www.addgene.org

Language: English - Date: 2014-11-05 23:32:03
122Molecular biology / Cre recombinase / Homologous recombination / Genetic recombination / Site-directed mutagenesis / Gene / Cre-Lox recombination / Recombineering / Biology / Genetics / Molecular genetics

Construct Design and Cloning Guide for Cas9-triggered homologous recombination Written by Dan Dickinson () and last updated DecemberReference: Dickinson DJ, Ward JD, Reiner DJ and Goldstein B

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Source URL: www.addgene.org

Language: English - Date: 2014-11-19 10:38:34
123Chemistry / Guide RNA / Polymerase chain reaction / Oligonucleotide / Molecular cloning / Vector / DNA microarray / Gene expression / Biology / Molecular biology / Biochemistry

gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’

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Source URL: www.addgene.org

Language: English - Date: 2014-11-05 23:32:09
124Chemistry / Polymerase chain reaction / Laboratory techniques / Amplifiers / Biotechnology / Dinoflagellate / Molecular cloning / Biology / Molecular biology / Biochemistry

Development of genetic probes for the identification of marine microalgae Progress Report, Part 2, June 2009 Paolo Momigliano, David Blair and Kirsten Heimann School of Marine and Tropical Biology, James Cook University

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Source URL: www.rrrc.com.au

Language: English - Date: 2009-11-11 23:25:09
125Gene / Molecular biology / Mouse / Europhenome / Biology / Genetics / Cloning

The German Mouse Clinic and INFRAFRONTIER: Examples from systemic phenotyping of genotypes and envirotypes in mouse models. Johannes Beckers, Martin Hrabé de Angelis and the GMC Consortium Since its opening in the year

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Source URL: www.towardsprecisionmedicine-symposium2014.org.au

Language: English - Date: 2014-09-30 17:29:24
126Chemistry / Guide RNA / Polymerase chain reaction / Oligonucleotide / Molecular cloning / Vector / DNA microarray / Gene expression / Biology / Molecular biology / Biochemistry

gRNA Synthesis Protocol STEP 1: Find all 23bp genomic sites of the form 5’-N20NGG-3’ near your intended target site (ideally ±50bp). These may reside on the + or – strand. PAM 5’!NNNNN NNNNN NNNNN NNNNN NGG!3’

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Source URL: www.addgene.org

Language: English - Date: 2014-11-05 23:32:02
127Polymerase chain reaction / Molecular cloning / Fluorescence in situ hybridization / Primer / Real-time polymerase chain reaction / Cloning / Biology / Molecular biology / Biochemistry

Short Scientific Report_MF_RIESCO A) Short scientific report Abstract The identification of vasa as the first marker in germ line in zebrafish (Danio rerio) involved, from the molecular point of view, a significant impr

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Source URL: aquagamete.webs.upv.es

Language: English - Date: 2014-12-18 06:38:38
128Cloning / Gene / Molecular biology / Classical genetics / Europhenome / SCO1 / Biology / Genetics / Medical genetics

NorCOMM2 – High-throughput knockout mouse production and phenotyping

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Source URL: www.towardsprecisionmedicine-symposium2014.org.au

Language: English - Date: 2014-07-07 19:00:05
129Chemistry / Polymerase chain reaction / Laboratory techniques / Biotechnology / Tropical diseases / Real-time polymerase chain reaction / Recombinant DNA / Reverse transcription polymerase chain reaction / Molecular cloning / Biology / Molecular biology / Biochemistry

SAMUEL HARRINGTON, Ph.D. VA Dept. of Health & Human Services Public Health Laboratories 6 Hazen Drive, Fairfax, VAOffice:

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Source URL: www.phoenixcareergroup.com

Language: English - Date: 2009-07-28 16:45:11
130Vector / Green fluorescent protein / Expression vector / Plasmid / Restriction enzyme / Multiple cloning site / Molecular cloning / Restriction digest / Gene expression / Biology / Molecular biology / Biochemistry

FireLab 2005 Mini-vector Kit July 2005 Documentation "alpha" version Direct contributors to making and testing vectors in this kit In our lab:

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Source URL: www.addgene.org

Language: English - Date: 2014-11-05 23:32:08
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